Preparative Electrophoresis for Plasmid DNA.
نویسندگان
چکیده
منابع مشابه
Continuous preparative gel electrophoresis.
Electrophoresis is widely used as a separative technique, particularly in protein chemistry. In some respects gelatinous media have an advantage over others as support media for electrophoresis, perhaps most obviously when a gel matrix of molecular dimensions is employed, allowing the simultaneous use of two properties for separation of the particles of interest-size and surface charge density....
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paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi ca...
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A method of preparative electrophoresis is described. A volume of approximately 1 ml. of protein solution can be applied with the cell used. A “marker” solution, applied adjacent to the sample to be separated, indicates the positions of the protein bands during the migration. The procedure has the advantages of a high degree of recovery, simplicity of operation, and utilization of an electropho...
متن کاملOptimum conditions for preparative operation of capillary zone electrophoresis
The possibilities to use capillary zone electrophoresis (CZE) as a preparative tool have been studied, mostly from a theoretical point of view. The preparative performance of CZE is quantitated by the production rate, the amount of analyte that can be purified per unit of time. The production rate available with CZE is shown to depend in the first place on the chemical characteristics of the an...
متن کاملProtection of DNA during preparative agarose gel electrophoresis against damage induced by ultraviolet light.
Preparative gel electrophoresis of double-stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in vitro transcription, transformation of E. coli and PCR. Inhibition was not caused by enzyme-inhibiting impurities in the agarose gel, but was i...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1991
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.7.517